Device

Part:BBa_K4194018:Design

Designed by: Jiong Huang   Group: iGEM22_BUCT-China   (2022-09-24)


p(Bla)-Lac operator-RBS-CI-LVA-RBS-EGFP-LVA-terminator-pRM'-RBS-LacI-LVA-RBS-mRFP1-LVA-terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2942
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2942
    Illegal NotI site found at 765
    Illegal NotI site found at 3841
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1617
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2942
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2942
    Illegal NgoMIV site found at 1649
    Illegal NgoMIV site found at 2458
    Illegal AgeI site found at 647
    Illegal AgeI site found at 2035
    Illegal AgeI site found at 3723
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization for Escherichia coli has been done.


Source

None

References